APP antibody (C-Term)

Catalog No. ABIN5596698

Supplier Product No.: 600-401-j54

Product Details anti-APP Antibody

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APP antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 600-401-j54

Supplier: Rockland

Purpose: Beta Amyloid 42 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest reactivity with human, sheep, rabbit, canine, bovine, pig, guinea pig, and monkey based on 100 % sequence homology.

Characteristics: Synonyms: rabbit anti-Beta Amyloid 42 Antibody, ß-amyloid 42, Amyloid beta A4 protein, Alzheimer disease amyloid protein, Beta amyloid, A-beta, ABPP, APPI, Alzheimer disease amyloid protein, Cerebral vascular amyloid peptide, Protease nexin-II

Purification: Anti-Beta Amyloid 42 was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Beta Amyloid 42 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus of AB42.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Beta Amyloid 1-42 antibody has been tested by ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~86 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1: 10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for APP

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Alternative Name: APP (APP Products)

Background: Background: Beta Amyloid functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. It is expressed in all fetal tissues with the highest levels located in the brain, kidney, heart and spleen tissue. It is involved in cell mobility, transcription regulation via protein-protein interactions and copper homeostasis/oxidative stress through copper ion reduction. The copper-metallated APP induces neuronal death directly in vitro, or is potentiated through Cu2+ mediated low-density lipoprotein oxidation. It has binding capabilities via its C-terminus for transient metals such as copper, zinc and iron. It binds APBB1-KAT5 to promote transcription activation and inhibits Notch signaling through interaction with Numb. It also promotes tau aggregation and TPK II-mediated phosphorylation. Anti-Beta Amyloid 42 regulates neurite outgrowth by binding components in the cellular matrix such as heparin, collagen I and amyloid-beta peptide, leading to mitochondrial dysfunction in cultured cortical neurons. Defects in APP cause Alzheimer disease type 1 and cerebral amyloid angiopathy. This antibody is ideal for researchers interested in Cancer or Neuroscience research.

NCBI Accession: NP_000475

UniProt: P05067

Pathways: Caspase Cascade in Apoptosis, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour