Bilirubin Oxidase antibody (HRP)
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- Target See all Bilirubin Oxidase products
- Bilirubin Oxidase
- Reactivity
- Myrothecium verrucaria
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This Bilirubin Oxidase antibody is conjugated to HRP
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Application
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- Cross-Reactivity (Details)
- Cross reactivity against Bilirubin Oxidase from other sources is unknown.
- Purification
- Bilirubin Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Bilirubin Oxidase [Myrothecium verrucaria].
- Immunogen
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Immunogen: Bilirubin Oxidase [Myrothecium verrucaria]
Immunogen Type: Native Protein
- Isotype
- IgG
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anti-Bilirubin Oxidase antibody (Biotin)
Reactivity: Myrothecium verrucaria WB, ELISA Host: Goat Polyclonal Biotin
anti-Bilirubin Oxidase antibodyReactivity: Myrothecium verrucaria Host: Goat Polyclonal unconjugated
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- Application Notes
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Application Note: Anti-Bilirubin Oxidase has been assayed against 1.0 μg of Bilirubin Oxidase [Myrothecium verrucaria] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,500 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:2,500
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:1,000 - 1:5,000
- Comment
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User Optimized
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- Preservative
- Gentamicin sulfate
- Precaution of Use
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- Bilirubin Oxidase
- Abstract
- Bilirubin Oxidase Products
- Synonyms
- Bilirubin oxidase antibody, bilirubin oxidase antibody, hypothetical protein antibody, Franean1_4816 antibody, Dole_0431 antibody, SARE_RS06935 antibody, PTRG_09905 antibody, Chy400_1070 antibody, Bcav_4005 antibody, Caci_0156 antibody, Hneap_0275 antibody, Sthe_0428 antibody, HTUR_RS21015 antibody, TK90_1924 antibody, BC1002_7184 antibody, Gobs_1113 antibody, Toce_1552 antibody, AciX9_0181 antibody, NAL212_2028 antibody, Psed_5122 antibody, Lbuc_2174 antibody, Halhy_6631 antibody, HALXA_RS20230 antibody
- Background
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Synonyms: Bilirubin Oxidase
Background: Bilirubin Oxidase is key in the oxidation of bilirubin and other tetrapyrroles. This enzyme belongs to the family of oxidoreductases, to be specific those acting on the CH-CH group of donor with oxygen as acceptor. The systematic name of this enzyme class is bilirubin:oxygen oxidoreductase. This enzyme is also called bilirubin oxidase M-1. This enzyme participates in porphyrin and chlorophyll metabolism.
Gene Name: BLRO_MYRVE
- UniProt
- Q12737
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