Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Calf Intestinal Alkaline Phosphatase (CIAP) antibody (HRP)

Rockland 200-6335-0100 CIAP Reactivity: Cow WB, DB Host: Sheep Polyclonal HRP
Rockland
Catalog No. ABIN5596690
Supplier Product No.: 200-6335-0100
  • Target See all Calf Intestinal Alkaline Phosphatase (CIAP) products
    Calf Intestinal Alkaline Phosphatase (CIAP)
    Reactivity
    Cow
    Host
    • 4
    • 2
    • 1
    Sheep
    Clonality
    • 7
    Polyclonal
    Conjugate
    • 4
    • 2
    • 1
    HRP
    Application
    • 7
    • 7
    • 4
    • 1
    Western Blotting (WB), Dot Blot (DB)
    Supplier Product No.
    200-6335-0100
    Supplier
    Rockland
    Purpose
    Alkaline Phosphatase Antibody Peroxidase Conjugated
    Cross-Reactivity (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Alkaline Phosphatase [Calf Intestine].
    Characteristics
    Synonyms: sheep anti-Alkaline Phosphatase HRP conjugated Antibody, peroxidase conjugated sheep anti-Alkaline Phosphatase Antibody, Alkaline phosphomonoesterase antibody, ALPI antibody, Glycerophosphatase antibody, IAP antibody, Intestinal alkaline phosphatase antibody, Kasahara isozyme antibody
    Purification
    Alkaline phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    Immunogen

    Immunogen: Alkaline Phosphatase [Calf Intestine]

    Immunogen Type: Native Protein

    Isotype
    IgG
  • Application Notes

    Application Note: Anti-Alkaline phosphatase peroxidase conjugated has been tested by dot blot and is suitable to be assayed against 1.0 μg of Alkaline Phosphatase [Calf Intestine] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:500 to 1:1,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:250

    Other: User Optimized

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
    Preservative
    Gentamicin sulfate
    Precaution of Use
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Expiry Date
    12 months
  • Target
    Calf Intestinal Alkaline Phosphatase (CIAP)
    Alternative Name
    Calf Intenstinal Alkaline Phosphatase (CIAP Products)
    Background
    Background: Alkaline phosphatase (ALP, ALKP) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. All mammalian alkaline phosphatase isoenzymes except placental (PALP and SEAP) are inhibited by homoarginine, and, in similar manner, all except the intestinal and placental ones are blocked by levamisole. Heating for ~2 hours at 65 °C inactivated most isoenzymes except Placental isoforms (PALP and SEAP).
    Gene ID
    280993
    UniProt
    P19111
You are here:
Support