This product has been assayed against 1.0 µg of Aldolase [Rabbit Muscle] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:2,000 of the reconstitution concentration is suggested for this product. ELISA Dilution: 1:5,000 - 1:20,000 Immunoprecipitation Dilution: 1:100 Western Blot Dilution: 1:500 - 1:5,000
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Restore with deionized water (or equivalent)
Concentration
10.0 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2