APOE antibody

Catalog No. ABIN5596681

Supplier Product No.: 600-101-197

Product Details anti-APOE Antibody

Target: APOE (Apolipoprotein E (APOE))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This APOE antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Supplier Product No.: 600-101-197

Supplier: Rockland

Purpose: APOLIPOPROTEIN E Antibody

Cross-Reactivity (Details): Typically less than 1 % cross-reactivity against other types of apoLipoprotein was detected by ELISA against purified standards.

Characteristics: Synonyms: goat anti-Apolipoprotein E3 antibody, Alzheimer disease 2 antibody, Apo-E antibody, Apo E antibody, ApoE antibody, APOEA antibody, Apolipoprotein-E antibody, Apoprotein antibody

Purification: Anti-Apolipoprotein E has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities.

Sterility: Sterile filtered

Immunogen:

Immunogen: Apolipoprotein Type E was isolated from human plasma by density gradient centrifugation followed by HPLC purification.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:50 - 1:200

Application Note: Apolipoprotein E antibodies have been tested by western blot and used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis.

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:4,000 - 1:8,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Expiry Date: 12 months

References for anti-APOE antibody (ABIN5596681)

Lu, Yuan, Wang, Zhang, Yuan, Wu, Zhou, He, Lu, Chen, Fan, Liang, Cheng: "Spontaneous severe hypercholesterolemia and atherosclerosis lesions in rabbits with deficiency of low-density lipoprotein receptor (LDLR) on exon 7." in: EBioMedicine, Vol. 36, pp. 29-38, (2019) (PubMed).

Target Details for APOE

Target: APOE (Apolipoprotein E (APOE))

Alternative Name: APOE (APOE Products)

Background: Background: Anti Apolipoprotein E Antibody recognizes the gene product of APOE. Apolipoprotein E is found in the chylomicron and IDLs that binds to a specific receptor on liver cells and peripheral cells. Apolipoprotein E is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This antibody is suitable for cardiovascular research.

Gene ID: 348

NCBI Accession: NP_000032

UniProt: P02649

Pathways: Regulation of Cell Size, Lipid Metabolism