SNCA antibody

Catalog No. ABIN5564053

Product Details anti-SNCA Antibody

Target: SNCA (Synuclein, alpha (SNCA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SNCA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Dot Blot (DB)

Specificity: Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.,Binds within the 81-130 aa region of human ASYN, determined using WB and ELISA with corresponding peptides. Detects ~14 kDa band corresponding to ASYN. Band at ~30 kDa is a dimer.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Human alpha synuclein monomer

Clone: 3C11

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• DB (1:1000)
• ICC/IF (1:100)
• ELISA (1:1000)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN5564053 was sufficient for detection of Alpha Synuclein in 15 μg of human brain cell lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

Target Details for SNCA

Target: SNCA (Synuclein, alpha (SNCA))

Alternative Name: Alpha Synuclein (SNCA Products)

Synonyms: snca antibody, MGC64356 antibody, LOC619283 antibody, NACP antibody, alphaSYN antibody, PARK1 antibody, PARK4 antibody, PD1 antibody, synuclein alpha L homeolog antibody, alpha-synuclein antibody, synuclein alpha antibody, synuclein, alpha antibody, snca.L antibody, LOC619283 antibody, SNCA antibody, Snca antibody

Background: Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally, it has been shown to significantly interact with tubulin (3), and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions, inactivation may lead to impaired spatial learning and working memory (4). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimers disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson's disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin (5, 6).

Gene ID: 6622

NCBI Accession: NP_000336

UniProt: P37840

Pathways: Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Platelet-derived growth Factor Receptor Signaling, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity