AAV VP1 antibody (AA 30-80) (AbBy Fluor® 750)
-
- Target See all AAV VP1 products
- AAV VP1 (Adeno-Associated Virus, VP1 (AAV VP1))
- Binding Specificity
- AA 30-80
- Reactivity
- Adeno-Associated Virus (AAV)
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This AAV VP1 antibody is conjugated to AbBy Fluor® 750
-
Application
- Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
- Specificity
- This antibody will recognize many AAV strains, including AAV5, AAV4, AAV3B, AAV9, and AAV13
- Cross-Reactivity
- Virus
- Cross-Reactivity (Details)
- AAV5
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide derived from Adeno-Associated Virus 5 capsid protein VP1
- Isotype
- IgG
-
-
- Application Notes
- IF(IHC-P) 1:50-200
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
-
- Target
- AAV VP1 (Adeno-Associated Virus, VP1 (AAV VP1))
- Alternative Name
- Adeno-Associated Virus Capsid Protein VP1 (AAV VP1 Products)
- Target Type
- Viral Protein
- Background
-
Synonyms: capsid protein Adeno-associated 5 virus, capsid protein [Adeno-associated virus - 5], capsid protein AAV5, Parvovirus coat protein VP1, capsid protein.
Background: Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of three size variants of the capsid protein VP1, VP2 and VP3 which differ in their N-terminus. The capsid encapsulates the genomic ssDNA. Binds to host cell heparan sulfate and uses host ITGA5-ITGB1 as coreceptor on the cell surface to provide virion attachment to target cell. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Binding to the host receptor also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and putative nuclear localization signal(s). VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell and might contribute to virus transport to the nucleus
-