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NRF2 antibody (pSer40)

Name: NRF2 antibody (pSer40)
SKU: ABIN5557501
Price: 490.77 USD
Availability: InStock

NFE2L2 Reactivity: Human, Mouse, Rat WB, IHC (p), FACS, IF (cc) Host: Rabbit Monoclonal 7G4 unconjugated

Lane 1: HepG2, Lane 2: Raji lysates probed with Nrf2(S40) (7G4) Monoclonal Antibody at 1:1000 overnight at 4˚C. (NRF2 antibody (pSer40))

HepG2 cells were stained with Nrf2(S40) (7G4) Monoclonal Antibody at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection. (NRF2 antibody (pSer40))

IF (cc)

A549 cells were stained with Nrf2(S40) (7G4) Monoclonal Antibody at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection. (NRF2 antibody (pSer40))

IF (cc)

Paraformaldehyde-fixed, paraffin embedded human tonsil section, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal serum) at 37°C for 20min, Antibody incubation with Nrf2(S40) (7G4) Monoclonal Antibody at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining. (NRF2 antibody (pSer40))

IHC (p)

4 images

Catalog No. ABIN5557501

Datasheet as PDF

Target See all NRF2 (NFE2L2) Antibodies

NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))
Binding Specificity
20

15

9

9

8

8

7

4

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer40
Reactivity
112

54

53

6

5

4

3

3

2

1

1

1

1

1
Human, Mouse, Rat
Host
104

10

1
Rabbit
Compatible Secondaries
Clonality
97

18
Monoclonal
Conjugate
56

10

6

6

4

3

3

3

3

3

3

3

1

1

1

1

1

1

1

1

1

1

1

1
This NRF2 antibody is un-conjugated
Application
87

50

24

21

16

15

14

14

12

9

7

2

2

2

1

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc))

Cross-Reactivity

Human, Mouse, Rat

Purification

Purified by Protein A.

Immunogen

Synthetic peptide derived from human Nrf2(S40), around 1-70aa (phospho S40).

Clone

7G4

Isotype

IgG

Top Product

Discover our top product NFE2L2 Primary Antibody
anti-Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) (pSer40) antibody
NFE2L2 Reactivity: Human, Mouse, Rat, Pig WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) (AA 430-480) antibody
NFE2L2 Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) (Internal Region) antibody
NFE2L2 Reactivity: Human, Rat WB, IF, IP, ICC, IHC (p), ChIP Host: Rabbit Polyclonal unconjugated

anti-Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) (AA 366-605) antibody
NFE2L2 Reactivity: Human WB, IHC, IF, IP Host: Rabbit Polyclonal unconjugated

anti-Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) (acLys599) antibody
NFE2L2 Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) (AA 382-604) antibody
NFE2L2 Reactivity: Rat WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) (N-Term) antibody
NFE2L2 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) (AA 356-589) antibody
NFE2L2 Reactivity: Human ELISA, IHC Host: Mouse Monoclonal 1A6G6 unconjugated

anti-Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) (AA 445-458) antibody
Verified NFE2L2 Reactivity: Human WB, ELISA, IF, FACS Host: Goat Polyclonal unconjugated

anti-Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) (AA 71-170) antibody
NFE2L2 Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 1B8 unconjugated

Application Notes

WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Target

NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))

Alternative Name

Nrf2 (NFE2L2 Products)

Synonyms

Nrf2 antibody, nfe2l2 antibody, wu:fc15g09 antibody, wu:fj67e03 antibody, NF-E2R2 antibody, NRF2 antibody, AI194320 antibody, nuclear factor, erythroid 2-like 2a antibody, nuclear factor, erythroid 2 like 2 S homeolog antibody, nuclear factor, erythroid 2 like 2 antibody, nuclear factor, erythroid derived 2, like 2 antibody, nuclear factor, erythroid 2-like 2 antibody, nfe2l2a antibody, nfe2l2.S antibody, NFE2L2 antibody, Nfe2l2 antibody

Background

Synonyms: NRF2, Nuclear factor erythroid 2-related factor 2, NF-E2-related factor 2, NFE2-related factor 2, HEBP1, Nuclear factor, erythroid derived 2, like 2, NFE2L2
Background: Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.

Gene ID

4780

UniProt

Q16236

Pathways

ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling