Histone H3.3 antibody

Catalog No. ABIN5557308

Product Details anti-Histone H3.3 Antibody

Target: Histone H3.3

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Histone H3.3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: Recombinant human Histone H3.3 protein, full length.

Clone: 7B12

Isotype: IgG

Application Details

Application Notes: WB 1:300-1000
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for Histone H3.3

Target: Histone H3.3

Abstract: Histone H3.3 Products

Synonyms: H3.3A antibody, H3F3 antibody, H3-IX antibody, H3-X antibody, H3.3B antibody, H3F3C antibody, H3F3B antibody, H3 histone family member 3A antibody, H3 histone, family 3A antibody, H3 histone, family 3B antibody, H3 histone, family 3C L homeolog antibody, histone H3.3 antibody, Histone H3.3 antibody, histone H3.3B-like antibody, H3F3A antibody, H3F3B antibody, H3f3a antibody, h3f3c.L antibody, LOC5567559 antibody, Bm1_29875 antibody, Bm1_40030 antibody, PTRG_03457 antibody, Phum_PHUM492720 antibody, UREG_07214 antibody, PAAG_07099 antibody, PITG_06950 antibody, PITG_06953 antibody, PITG_06955 antibody, LOC100361558 antibody, LOC100521680 antibody

Background:

Synonyms: H3F3, H3.3A, Histone H3.3, H3F3A, PP781

Background: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Gene ID: 3020

UniProt: P84243