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Ezrin antibody (AA 292-464)

Name: Ezrin antibody (AA 292-464)
Brand: antibodies-online
SKU: ABIN5542456
Price: 511.5 USD
Availability: InStock

EZR Reactivity: Human WB, ELISA, ICC, FACS Host: Mouse Monoclonal 6F1A9 unconjugated

Western blot analysis using EZR mAb against human EZR (AA: 292-464) recombinant protein. (Ezrin antibody (AA 292-464))

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng), (Ezrin antibody (AA 292-464))

ELISA

Western blot analysis using EZR mAb against HEK293 (1) and EZR (AA: 292-464)-hIgGFc transfected HEK293 (2) cell lysate. (Ezrin antibody (AA 292-464))

Western blot analysis using EZR mouse mAb against MCF-7 (1), Hela (2), A431 (3), Hek293 (4), SK-N-SH (5), Jurkat (6), HepG2 (7), NIH/3T3 (8), and Cos7 (9) cell lysate. (Ezrin antibody (AA 292-464))

Flow cytometric analysis of MCF-7 cells using EZR mouse mAb (green) and negative control (red). (Ezrin antibody (AA 292-464))

FACS

Flow cytometric analysis of Hela cells using EZR mouse mAb (green) and negative control (red). (Ezrin antibody (AA 292-464))

FACS

Immunofluorescence analysis of A549 cells using EZR mouse mAb (green). (Ezrin antibody (AA 292-464))

7 images

(1 reference)

Catalog No. ABIN5542456

Datasheet as PDF

Target See all Ezrin (EZR) Antibodies

Ezrin (EZR)
Binding Specificity
28

16

13

11

10

9

9

8

5

5

5

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 292-464
Reactivity
157

84

75

9

8

6

6

4

4

3

3

1

1

1

1
Human
Host
148

8

3

2
Mouse
Compatible Secondaries
Clonality
145

15
Monoclonal
Conjugate
93

8

5

5

5

5

5

5

4

3

2

2

2

2

2

2

2

2

2

2

2

1
This Ezrin antibody is un-conjugated
Application
121

58

26

15

15

13

13

12

10

7

3

2

1

1

1

1

1

1

1
Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Purpose

EZR Antibody

Purification

Purified antibody

Immunogen

Purified recombinant fragment of human EZR (AA: 292-464) expressed in E. Coli.

Clone

6F1A9

Isotype

IgG1
anti-Ezrin (EZR) (AA 1-295) antibody
EZR Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Ezrin (EZR) (pTyr478) antibody
EZR Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Ezrin (EZR) (AA 1-586) antibody
EZR Reactivity: Human WB, IP Host: Rabbit Polyclonal unconjugated

anti-Ezrin (EZR) (pTyr353) antibody
EZR Reactivity: Human, Mouse, Rat WB, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Ezrin (EZR) antibody
EZR Reactivity: Human WB, IHC, StM Host: Mouse Monoclonal CPTC-Ezrin-1 unconjugated

anti-Ezrin (EZR) (AA 1-586) antibody
EZR Reactivity: Human WB, ELISA, IHC (p), PLA Host: Mouse Monoclonal 1E11 unconjugated

anti-Ezrin (EZR) (AA 350-470) antibody
EZR Reactivity: Human WB, IP, IF Host: Rabbit Polyclonal unconjugated

anti-Ezrin (EZR) (AA 1-251) antibody
EZR Reactivity: Human ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Ezrin (EZR) (AA 455-586) antibody
EZR Reactivity: Human, Rat WB, ELISA, IHC (p), FACS, IF (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Ezrin (EZR) (C-Term) antibody
EZR Reactivity: Human, Mouse, Rat WB, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

Application Notes

ELISA: 1/10000
FCM: 1/200 - 1/400
ICC: 1/100 - 1/400

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified antibody in PBS with 0.05 % sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Popov, Brazhe, Fedotova, Tiaglik, Bychkov, Morozova, Brazhe, Aronov, Lyukmanova, Lazareva, Li, Ponimaskin, Verkhratsky, Semyanov: "A high-fat diet changes astrocytic metabolism to promote synaptic plasticity and behavior." in: Acta physiologica (Oxford, England), pp. e13847, (2022) (PubMed).
Target

Ezrin (EZR)

Alternative Name

EZR (EZR Products)

Synonyms

CVIL antibody, CVL antibody, VIL2 antibody, ezr antibody, vil2 antibody, wu:fc05c03 antibody, wu:fj31d06 antibody, cvil antibody, cvl antibody, Vil2 antibody, AW146364 antibody, R75297 antibody, p81 antibody, ezr.S antibody, ezrin antibody, ezrin b antibody, ezrin L homeolog antibody, EZR antibody, ezrb antibody, ezr antibody, Ezr antibody, ezr.L antibody

Background

Description: The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.
Aliases: CVL, CVIL, VIL2, HEL-S-105

Molecular Weight

69.4kDa

Gene ID

7430

HGNC

7430

UniProt

P15311

Pathways

Maintenance of Protein Location