ADH1 antibody (Biotin)

Catalog No. ABIN5541710

Product Details anti-ADH1 Antibody (Biotin)

Target: ADH1 (Alcohol Dehydrogenase 1 (Class I) (ADH1))

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADH1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Specificity: This antibody detects yeast alcohol dehydrogenase. Cross reactivity against alcohol dehydrogenase from other sources may occur but have not been specifically determined. Immunoelectrophoresis gives a single precipitin arc against anti-Biotin, anti-rabbit serum as well as purified and partially purified yeast alcohol dehydrogenase.

Purification: Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer

Immunogen: Alcohol dehydrogenase from yeast

Application Details

Application Notes: Western blot : 1/500-1/5,000. Immunoprecipitation : 1/100. ELISA : 1/5,000-1/20,000. Antibody has been assayed against 1.0 μg of Alcohol dehydrogenase in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/20,000 of the reconstitution concentration is suggested.

Restrictions: For Research Use only

Handling

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for ADH1

Target: ADH1 (Alcohol Dehydrogenase 1 (Class I) (ADH1))

Alternative Name: alcohol dehydrogenase 1 (ADH1 Products)

Synonyms: ADH-AA antibody, AI194826 antibody, Adh-1 antibody, Adh-1-t antibody, Adh-1e antibody, Adh-1t antibody, Adh-3e antibody, Adh1-e antibody, Adh1-t antibody, Adh1tl antibody, Adh3-e antibody, Adh antibody, Adh1a antibody, Adh1c antibody, ADH3 antibody, ADH-1 antibody, ADH1 antibody, ADH1B antibody, alcohol dehydrogenase 1 (class I) antibody, alcohol dehydrogenase 1C (class I), gamma polypeptide antibody, alcohol dehydrogenase 1A (class I), alpha polypeptide antibody, Adh1 antibody, ADH1C antibody, ADH1A antibody

UniProt: P00330