beta-Galactosidase Tag antibody (Biotin)
WB, EIA, IHC (p), IHC (fro)
Host: Rabbit
Polyclonal
Biotin
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- Target See all beta-Galactosidase Tag products
- beta-Galactosidase Tag
- Reactivity
- Please inquire
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This beta-Galactosidase Tag antibody is conjugated to Biotin
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificity
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum as well as purified and partially purified Beta-galactosidase (E.coli).
- Purification
- Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
- Immunogen
- Beta-galactosidase from E.coli
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- Application Notes
- Western blot : 1/2,000-1/10,000. ELISA : 1/10,000-1/50,000. IHC : 1/1,000-1/5,000. The antibody recognizes both frozen tissue sections, paraffin embedded tissue and 4 % paraformaldehyde fixed tissue for most immunohistochemical analysis : A 1:1,500 dilution has been reported to detect beta-galactosidase in adult rat spinal cord tissue after infection with helper-dependent adenovirus expressing lacZ. In this particular experiment, tissue was perfused with 4 % paraformaldehyde and cryostat-cut (35 μm) to produce free-floating sections. A 1:5,000 dilution has been reported to be successful for staining of brain sections from transgenic mice expressing nuclear beta-galactosidase when assayed by immunofluorescence microscopy. A 1:5,000 dilution has been reported for immunofluorescent staining of methanol fixed, devitellinized Drosophila embryos. A 1:5,000 dilution has been reported to be successful for staining by immunoblot of beta-galactosidase fusion proteins after transfer using a semi-dry transfer apparatus.
- Restrictions
- For Research Use only
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- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
- Expiry Date
- 12 months
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- Target
- beta-Galactosidase Tag
- Abstract
- beta-Galactosidase Tag Products
- Target Type
- Tag
- UniProt
- P00722
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