CEACAM5/6 antibody

Catalog No. ABIN5541175

Product Details anti-CEACAM5/6 Antibody

Target: CEACAM5/6 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5/6 (CEACAM5/6))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CEACAM5/6 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Enzyme Immunoassay (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: This antibody reacts to CD66c/e. Species: Human.

Purification: Affinity Chromatography on Protein G

Immunogen: MUS was generated by immunisation of BALB/c mice with CEA partially purified from a perchloric acid extract from liver metastases of colonic tumors (3).

Clone: MUS

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 1.2 μg/10 6 cells. ELISA: 1/200-1/400. Cell based ELISA with intakt, transiently transfected cells: 1/200. Western blot: 4 μg/mL. Immunohistochemistry: 1-2 μg/10 cells (on Cryosections).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Phosphate buffered saline, pH 7.2

Storage: 4 °C,-20 °C

Storage Comment: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

References for anti-CEACAM5/6 antibody (ABIN5541175)

Voges, Bachmann, Naujoks, Kopp, Hauck: "Extracellular IgC2 constant domains of CEACAMs mediate PI3K sensitivity during uptake of pathogens." in: PLoS ONE, Vol. 7, Issue 6, pp. e39908, (2012) (PubMed).

Tsutsumida, Swanson, Singh, Caffrey, Kitajima, Goto, Yonezawa, Hollingsworth: "RNA interference suppression of MUC1 reduces the growth rate and metastatic phenotype of human pancreatic cancer cells." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 12, Issue 10, pp. 2976-87, (2006) (PubMed).

Target Details for CEACAM5/6

Target: CEACAM5/6 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5/6 (CEACAM5/6))

Alternative Name: cd66c/e (CEACAM5/6 Products)

Synonyms: CEACAM5 antibody, CEA antibody, carcinoembryonic antigen related cell adhesion molecule 5 antibody, carcinoembryonic antigen-related cell adhesion molecule 5 antibody, CEACAM5 antibody

Background: CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family (1). The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/CEACAM5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker (2). CEACAM6 expression is strongly upregulated already during early stages of adenocarcinoma formation (3). The function of CEA family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria (1).