This Beta-Phosphoglucomutase antibody is conjugated to HRP
Application
Western Blotting (WB), Enzyme Immunoassay (EIA)
Specificity
This antibody detects Beta-phosphoglucomutase from Lactococcus lacti. Cross reactivity against Beta-phosphoglucomutase from other sources is unknown. Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-goat serum as well as purified and partially purified beta-phosphoglucomutase [ Lactococcus lacti ].
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Immunogen
Beta-phosphoglucomutase (PGMB) from Lactococcus lacti
Reactivity: Lactococcus lacti
WB, EIA, IP
Host: Goat
Polyclonal
unconjugated
Application Notes
Western blot : 1/1,000-1/5,000. ELISA : 1/10,000-1/40,000 dilution suggested to begin optimization. This product has been assayed against 1.0 μg of Beta-phosphoglucomutase [ Lactococcus lacti ] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/5,000 to 1/20,000 of the reconstitution concentration is suggested.
Restrictions
For Research Use only
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.