Beta-Phosphoglucomutase antibody (Biotin)

Catalog No. ABIN5540026

Product Details anti-Beta-Phosphoglucomutase Antibody (Biotin)

Target: Beta-Phosphoglucomutase (Phosphoglucomutase, beta-)

Reactivity: Lactococcus lacti

Host: Goat

Clonality: Polyclonal

Conjugate: This Beta-Phosphoglucomutase antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody detects Beta-phosphoglucomutase from Lactococcus lacti. Cross reactivity against Beta-phosphoglucomutase from other sources is unknown. Immunoelectrophoresis give a single precipitin arc against anti-biotin, anti-goat serum as well as purified and partially purified beta-phosphoglucomutase [ Lactococcus lacti ].

Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer

Immunogen: Beta-phosphoglucomutase (PGMB) from Lactococcus lacti

Application Details

Application Notes: Western blot : 1/500-1/3000. ELISA : 1/5,000-1/25,000 dilution suggested to begin optimization. This product has been assayed against 1.0 μg of Beta-phosphoglucomutase [ Lactococcus lacti ] in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/10,000 to 1/50,000 of the reconstitution concentration is suggested.

Restrictions: For Research Use only

Handling

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Beta-Phosphoglucomutase

Target: Beta-Phosphoglucomutase (Phosphoglucomutase, beta-)

Alternative Name: pgmb (Beta-Phosphoglucomutase Products)

Synonyms: ECK1312 antibody, JW1310 antibody, beta-phosphoglucomutase antibody, ycjU antibody, pgmB antibody, LMOf2365_2822 antibody, Exig_2478 antibody, Hore_00430 antibody, Tmath_0800 antibody, Aazo_0214 antibody, Spirs_3639 antibody, Closa_0065 antibody, Toce_1769 antibody, Tthe_1054 antibody, Lbys_0589 antibody, Celal_0979 antibody, Sgly_0722 antibody, Celly_2426 antibody, Spico_1537 antibody, Theth_1551 antibody

UniProt: P71447