FH antibody (Internal Region) (Biotin)

Catalog No. ABIN5539935

Product Details anti-FH Antibody (Biotin)

Target: FH (Fumarate Hydratase (FH))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This FH antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Enzyme Immunoassay (EIA)

Purpose: Fumarase / FH, Biotinylated

Sequence: HPNDHVNKSQ SSND.

Predicted Reactivity: Human, Mouse, Rat, Dog

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

DS WB Results: Approx 48 kDa band observed in Human Kidney lysates (calculated MW of 54.6 kDa according to NP_000134.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for FH

Target: FH (Fumarate Hydratase (FH))

Alternative Name: Fumarate Hydratase (FH Products)

Background: FH, fumarate hydratase, FMRD, HLRCC, LRCC, MCL, MCUL1, fumarase

Gene ID: 2271

NCBI Accession: NP_000134