MEX3C antibody (AA 541-554)

Catalog No. ABIN5539884

Product Details anti-MEX3C Antibody

Target: MEX3C (Mex-3 Homolog C (C. Elegans) (MEX3C))

Binding Specificity: AA 541-554

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This MEX3C antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: MEX3C (aa541-554)

Sequence: TPRLSPTFPE SIEH

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.

Western Blot: Approx 60+36 kDa bands observed in Mouse Testis lysates, and these bands reduced after knock-down by gene trap (calculated MW of 69.4 kDa according to NP_057710.3). Recommended concentration: 0.2-0.5 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MEX3C

Target: MEX3C (Mex-3 Homolog C (C. Elegans) (MEX3C))

Alternative Name: MEX3C (MEX3C Products)

Background: MEX3C, mex-3 homolog C (C. elegans), FLJ38871, MEX-3C, RKHD2, RNF194, OTTHUMP00000163545, RING finger and KH domain-containing protein 2, RING finger protein 194, RNA-binding protein MEX3C, ring finger and KH domain containing 2

Gene ID: 51320, 240396, 307271

NCBI Accession: NP_057710