ORC4 antibody (C-Term) (Biotin)

Catalog No. ABIN5539875

Product Details anti-ORC4 Antibody (Biotin)

Target: ORC4 (Origin Recognition Complex, Subunit 4 (ORC4))

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This ORC4 antibody is conjugated to Biotin

Application: ELISA, Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Purpose: ORC4L, Biotinylated

Sequence: DVRQWATSSL SWL.

Specificity: This antibody is expected to recognize all reported isoforms (NP_002543.2, NP_001177810.1, NP_001177811.1). Reported variants represent identical protein: XP_011509557.1, NP_002543.2, NP_001177808.1, NP_859526.1, NP_859525.1 Reported variants represent id

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 48 kDa band observed in Jurkat nuclear lysates (calculated MW of 50.4 kDa according to NP_002543.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Chromatin immunoprecipitation: reported by a customer to work very well in ChIP.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ORC4

Target: ORC4 (Origin Recognition Complex, Subunit 4 (ORC4))

Alternative Name: ORC4 (ORC4 Products)

Synonyms: ORC4L antibody, ORC4P antibody, Orc4P antibody, Orc4l antibody, mMmORC4 antibody, CG2917 antibody, DmORC4 antibody, Dmel\\CG2917 antibody, ORC antibody, ORC4 antibody, dmOrc4 antibody, orc4 antibody, rDmORC antibody, orc4l antibody, fc50c03 antibody, wu:fc50c03 antibody, zgc:85772 antibody, ATORC4 antibody, F23H14.9 antibody, F23H14_9 antibody, ORIGIN RECOGNITION COMPLEX SUBUNIT 4 antibody, origin recognition complex subunit 4 antibody, origin recognition complex subunit 4 antibody, origin recognition complex, subunit 4 antibody, Origin recognition complex subunit 4 antibody, origin recognition complex subunit 4 L homeolog antibody, origin recognition complex subunit Orc4 antibody, ORC4 antibody, Orc4 antibody, orc4.L antibody, orc4 antibody, CNM01830 antibody

Background: ORC4, origin recognition complex subunit 4, ORC4L, ORC4P, origin recognition complex, subunit 4 homolog

Gene ID: 5000, 26428, 295596

NCBI Accession: NP_002543, NP_001177810, NP_001177811

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA