MIA antibody (Internal Region)

Catalog No. ABIN5539869

Product Details anti-MIA Antibody

Target: MIA (Melanoma Inhibitory Activity (MIA))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MIA antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Purpose: MIA / CD-RAP

Sequence: PKLADRKLCA DQE

Specificity: The immunizing peptide represents the N terminus of the mature protein. Reported variants represent identical protein: NP_006524.1, NP_001189482.1,

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen and Skeletal Muscle. Recommended concentration: 5 μg/mL.

Western Blot: Approx 14 kDa band observed in Human Glioma lysates (calculated MW of 14.5 kDa according to NP_006524.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MIA

Target: MIA (Melanoma Inhibitory Activity (MIA))

Alternative Name: MIA (MIA Products)

Synonyms: CD-RAP antibody, Mia1 antibody, Cdrap antibody, zgc:194157 antibody, mia antibody, CDRAP antibody, Fdp antibody, MIA antibody, MIAL antibody, melanoma inhibitory activity antibody, otoraplin antibody, cilia and flagella associated protein 100 antibody, MIA antibody, Mia antibody, mia antibody, Otor antibody, CFAP100 antibody

Background: MIA, melanoma inhibitory activity, CD-RAP, melanoma-derived growth regulatory protein

Gene ID: 8190

NCBI Accession: NP_006524