MDH2 antibody (AA 221-232)

Catalog No. ABIN5539865

Product Details anti-MDH2 Antibody

Target: MDH2 (Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2))

Binding Specificity: AA 221-232

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MDH2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: MDH2 (aa221-232)

Sequence: DQLTALTGRI QE

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 5 μg/mL.

Western Blot: Approx 36-37 kDa band observed in lysates of cell lines HeLa, HepG2 and K562, and approx 35 kDa band observed in Human Heart and Liver lysates (calculated MW of 35.5 kDa according to NP_005909.2). Recommended concentration: 0.01-0.03 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MDH2

Target: MDH2 (Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2))

Alternative Name: MDH2 (MDH2 Products)

Background: MDH2, malate dehydrogenase 2, NAD (mitochondrial), M-MDH, MDH, MGC:3559, MOR1, malate dehydrogenase, mitochondrial

Gene ID: 4191

NCBI Accession: NP_005909