Fibrillarin antibody (AA 99-110)
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- Target See all Fibrillarin (FBL) Antibodies
- Fibrillarin (FBL)
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Binding Specificity
- AA 99-110
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This Fibrillarin antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Purpose
- fibrillarin / FBL (aa99-110)
- Sequence
- CRGKEDALVT KN
- Cross-Reactivity
- Cow, Dog, Human, Mouse, Pig, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Isotype
- IgG
- Top Product
- Discover our top product FBL Primary Antibody
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anti-Fibrillarin (FBL) antibody
FBL Reactivity: Human WB, IF, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Fibrillarin (FBL) (AA 100-321) antibodyFBL Reactivity: Human WB, IF, IP Host: Rabbit Polyclonal unconjugated
anti-Fibrillarin (FBL) antibodyFBL Reactivity: Human WB, IF Host: Rabbit Monoclonal unconjugated
anti-Fibrillarin (FBL) antibodyFBL Reactivity: Saccharomyces cerevisiae WB, IF Host: Mouse Monoclonal 38F3 unconjugated
anti-Fibrillarin (FBL) (AA 1-321) antibodyFBL Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 4G9-E4 unconjugated
anti-Fibrillarin (FBL) (N-Term) antibodyFBL Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Guinea Pig, Rabbit WB, IHC Host: Rabbit Polyclonal unconjugated
anti-Fibrillarin (FBL) (Internal Region) antibodyFBL Reactivity: Human, Mouse, Rat WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-Fibrillarin (FBL) (AA 61-110) antibodyFBL Reactivity: Human, Mouse, Rat, Dog, Cow, Monkey, Horse, Guinea Pig WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Fibrillarin (FBL) (N-Term) antibodyFBL Reactivity: Human, Mouse, Rat, Dog WB, IHC Host: Rabbit Polyclonal unconjugated
anti-Fibrillarin (FBL) antibodyFBL Reactivity: Saccharomyces cerevisiae WB, IHC, IF, ICC Host: Mouse Monoclonal 38F3 unconjugated
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- Application Notes
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Western Blot: Approx 35 kDa band observed in lysates of cell line HEK293 (calculated MW of 33.8 kDa according to NP_001427.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:128000.
- Comment
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Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- Fibrillarin (FBL)
- Alternative Name
- FBL (FBL Products)
- Background
- FBL, fibrillarin, FIB, FLRN, RNU3IP1, 34 kDa nucleolar scleroderma antigen, 34-kD nucleolar scleroderma antigen, RNA, U3 small nucleolar interacting protein 1, rRNA 2'-O-methyltransferase fibrillarin
- Gene ID
- 2091, 14113, 292747
- NCBI Accession
- NP_001427
- Pathways
- Ribonucleoside Biosynthetic Process
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