STAT5A antibody (AA 681-692)

Catalog No. ABIN5539582

Product Details anti-STAT5A Antibody

Target: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Binding Specificity: AA 681-692

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This STAT5A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Purpose: STAT5A (aa681-692)

Sequence: KYYTPVLAKA VD

Specificity: This antibody is not expected to cross-react with STAT5B.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 5 μg/mL.

Western Blot: Approx 100 kDa band observed in lysates of cell line K562 (calculated MW of 90.6 kDa according to NP_003143.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for STAT5A

Target: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Alternative Name: STAT5A (STAT5A Products)

Synonyms: MGF antibody, STAT5 antibody, AA959963 antibody, Stat5 antibody, STAT5A/MGF antibody, STATA5 antibody, STAT5A antibody, stat5 antibody, stat5b antibody, signal transducer and activator of transcription 5A antibody, signal transducer and activator of transcription 5a antibody, STAT5A antibody, Stat5a antibody, stat5a antibody, LOC100348518 antibody, LOC100716811 antibody

Background: STAT5A, signal transducer and activator of transcription 5A, MGF, STAT5

Gene ID: 6776, 20850, 24918

NCBI Accession: NP_003143

Pathways: JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation