STAT5A antibody (AA 681-692)

Catalog No. ABIN5539582

Product Details anti-STAT5A Antibody

Target: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Binding Specificity: AA 681-692

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This STAT5A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Purpose: STAT5A (aa681-692)

Sequence: KYYTPVLAKA VD

Specificity: This antibody is not expected to cross-react with STAT5B.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 5 μg/mL.

Western Blot: Approx 100 kDa band observed in lysates of cell line K562 (calculated MW of 90.6 kDa according to NP_003143.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for STAT5A

Target: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Alternative Name: STAT5A (STAT5A Products)

Background: STAT5A, signal transducer and activator of transcription 5A, MGF, STAT5

Gene ID: 6776, 20850, 24918

NCBI Accession: NP_003143

Pathways: JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation