HLA-B antibody (Internal Region)
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- Target See all HLA-B Antibodies
- HLA-B (Major Histocompatibility Complex, Class I, B (HLA-B))
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Binding Specificity
- Internal Region
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This HLA-B antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Purpose
- HLA-B
- Sequence
- DRNTQIYKAQ AQT
- Cross-Reactivity
- Human
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Isotype
- IgG
- Top Product
- Discover our top product HLA-B Primary Antibody
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- Application Notes
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Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 5 μg/mL.
Western Blot: Approx 40+45 kDa bands observed in lysates of cell line Jurkat (calculated MW of 40.5 kDa according to NP_005505.2). The observed 45 kDa band represents the glycosylated form. Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 h
Peptide ELISA: antibody detection limit dilution 1:64000.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- HLA-B (Major Histocompatibility Complex, Class I, B (HLA-B))
- Alternative Name
- HLA-B (HLA-B Products)
- Synonyms
- AS antibody, HLAB antibody, SPDA1 antibody, mhc1b antibody, major histocompatibility complex, class I, B antibody, major histocompatibility complex nonclassical class Ib L homeolog antibody, HLA-B antibody, BOLA-B antibody, MAMU-B18 antibody, DKFZP469N1225 antibody, mhc1b.L antibody
- Background
- HLA-B, major histocompatibility complex, class I, B, AS, HLAB, SPDA1, HLA class I histocompatibility antigen, B alpha chain, MHC Class I HLA heavy chain, MHC HLA-B cell surface glycoprotein, MHC HLA-B transmembrane glycoprotein, MHC class I antigen GN0010
- Gene ID
- 3106
- NCBI Accession
- NP_005505
- Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
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