HSPD1 antibody (AA 333-344)

Catalog No. ABIN5539538

Product Details anti-HSPD1 Antibody

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Binding Specificity: AA 333-344

Reactivity: Human, Rat, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This HSPD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Purpose: HSP60 / HSPD1 (aa333-344)

Sequence: NLEDVQPHDL GK

Specificity: Reported variants represent identical protein: NP_955472.1, NP_002147.2.

Cross-Reactivity: Cow, Dog, Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.

Western Blot: Approx 60 kDa band observed in Human and Pig Heart, Kidney and Liver and in Rat Kidney lysates (calculated MW of 61.1 kDa according to Human NP_002147.2 and 60.9 kDa according to Pig NP_001241645.1 and Rat NP_071565.2 ). Recommended concentrat

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the mitochondria of HeLa cells and in the cytoplasm of A431 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for HSPD1

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Alternative Name: HSPD1 (HSPD1 Products)

Background: HSPD1, heat shock 60 kDa protein 1 (chaperonin), CPN60, GROEL, HLD4, HSP-60, HSP60, HSP65, HuCHA60, SPG13, 60 kDa chaperonin, 60 kDa heat shock protein, mitochondrial, P60 lymphocyte protein, chaperonin 60, heat shock protein 65, mitochondrial matrix prote

Gene ID: 3329

NCBI Accession: NP_002147

Pathways: Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity