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HMBS antibody (AA 160-173)

Verified HMBS Reactivity: Human WB, ELISA, IHC Host: Goat Polyclonal unconjugated
Catalog No. ABIN5539536
  • Target See all HMBS Antibodies
    HMBS (Hydroxymethylbilane Synthase (HMBS))
    Binding Specificity
    • 8
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 160-173
    Reactivity
    • 61
    • 36
    • 36
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Host
    • 56
    • 5
    Goat
    Clonality
    • 48
    • 13
    Polyclonal
    Conjugate
    • 26
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This HMBS antibody is un-conjugated
    Application
    • 39
    • 13
    • 10
    • 7
    • 7
    • 5
    • 4
    • 4
    • 4
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Purpose
    HMBS (aa160-173)
    Sequence
    HLEFRSIRGN LNTR
    Specificity
    This antibody is expected to recognize all reported isoforms (NP_000181.2, NP_001019553.1, NP_001245137.1, NP_001245138.1).
    Cross-Reactivity
    Human
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Isotype
    IgG
    Top Product
    Discover our top product HMBS Primary Antibody
  • Application Notes

    Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 5 μg/mL.

    Western Blot: Approx 40 kDa band observed in lysates of cell line K562 (calculated MW of 39.3 kDa according to NP_000181.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.

    Peptide ELISA: antibody detection limit dilution 1:32000.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    HMBS (Hydroxymethylbilane Synthase (HMBS))
    Alternative Name
    HMBS (HMBS Products)
    Background
    HMBS, hydroxymethylbilane synthase, PBG-D, PBGD, PORC, UPS, porphobilinogen deaminase, porphyria, acute, Chester type, pre-uroporphyrinogen synthase, uroporphyrinogen I synthase, uroporphyrinogen I synthetase
    Gene ID
    3145
    NCBI Accession
    NP_000181, NP_001019553, NP_001245137, NP_001245138
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