HDAC1 antibody (C-Term)

Catalog No. ABIN5539534

Product Details anti-HDAC1 Antibody

Target: HDAC1 (Histone Deacetylase 1 (HDAC1))

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This HDAC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP)

Purpose: Histone Deacetylase 1

Sequence: KPEAKGVKEE VK

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Western Blot: Appox 55 kDa band observed in Human Duodenum and Lymph Node lysates and approx 70 kDa band observed in lysates of cell lines HeLa and NIH3T3 (calculated MW of 55.1 kDa according to Human NP_004955.2 and Mouse NP_032254.1). The observed 70 kDa

Peptide ELISA: antibody detection limit dilution 1:4000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nucleus of NIH3T3 cells. Recommended concentration: 10μg/ml

Chromatin Immunoprecipitation: A 3.5-fold increased presence of H3 onto the Human P21 l

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for HDAC1

Target: HDAC1 (Histone Deacetylase 1 (HDAC1))

Alternative Name: HDAC1 (HDAC1 Products)

Background: HDAC1, histone deacetylase 1, DKFZp686H12203, GON-10, HD1, RPD3, RPD3L1 , reduced potassium dependency, yeast homolog-like 1

Gene ID: 3065

NCBI Accession: NP_004955

Pathways: Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Mitotic G1-G1/S Phases, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Negative Regulation of intrinsic apoptotic Signaling, Embryonic Body Morphogenesis