CYB5R3 antibody (C-Term)

Catalog No. ABIN5539503

Product Details anti-CYB5R3 Antibody

Target: CYB5R3 (Cytochrome B5 Reductase 3 (CYB5R3))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CYB5R3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: CYB5R3 / Dia 1

Sequence: CLPNLDHVGH PTER

Specificity: This antibody is expected to recognize all three reported isoforms (NP_000389.1, NP_001123291.1, NP_001165131.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Adrenal Gland shows heavy vesicular/granular staining in the cytoplasm of medular cells. Recommended concentration, 3-5 μg/mL.

Western Blot: Approx 37 kDa band observed in Human Placenta, Testis and Umbilical Cord lysates (calculated MW of 38.2 kDa according to NP_001165131.1). Recommended concentration: 0.01-0.03 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CYB5R3

Target: CYB5R3 (Cytochrome B5 Reductase 3 (CYB5R3))

Alternative Name: CYB5R3 (CYB5R3 Products)

Background: CYB5R3, cytochrome b5 reductase 3, B5R, DIA1, NADH-cytochrome b5 reductase 3, NADH-cytochrome b5 reductase 3 membrane-bound form, NADH-cytochrome b5 reductase 3 soluble form, OTTHUMP00000198574, OTTHUMP00000198665, diaphorase-1

Gene ID: 1727

NCBI Accession: NP_000389, NP_001123291, NP_001165131

Pathways: SARS-CoV-2 Protein Interactome