Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This SPRR1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-89 amino acids from the C-terminal region of human SPRR1B.
SPRR1B
Reactivity: Human
ELISA, IHC, WB
Host: Rabbit
Polyclonal
FITC
Application Notes
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Restrictions
For Research Use only
Format
Liquid
Concentration
0.496 mg/mL
Buffer
Supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CORNIFIN antibody, GADD33 antibody, SPRR1 antibody, SPRR1B antibody, small proline rich protein 1B antibody, small proline-rich protein antibody, small proline-rich protein 1B antibody, SPRR1B antibody, SPRP antibody, Sprr1b antibody
Background
SPRR1B is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. All that results in the formation of an insoluble envelope beneath the plasma membrane. Can function as both amine donor and acceptor in transglutaminase-mediated cross-linkage.