This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This UBE2H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 153-183 amino acids from the C-terminal region of human UBE2H.
UBE2H
Reactivity: Human
WB, IF (p), IF (cc)
Host: Rabbit
Polyclonal
AbBy Fluor® 488
Application Notes
For WB starting dilution is: 1:1000
Restrictions
For Research Use only
Format
Liquid
Concentration
0.42 mg/mL
Buffer
Supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2H is a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100 % identical to the mouse homolog and 98 % identical to the frog and zebrafish homologs, which indicates that this enzyme is highly conserved in eukaryotes.