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SIGLEC5 antibody (C-Term)

SIGLEC5 Reactivity: Human, Mouse WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN5534705
  • Target See all SIGLEC5 Antibodies
    SIGLEC5 (Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5))
    Binding Specificity
    • 9
    • 6
    • 6
    • 6
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    AA 482-513, C-Term
    Reactivity
    • 52
    • 25
    • 3
    Human, Mouse
    Host
    • 47
    • 5
    • 3
    Rabbit
    Clonality
    • 47
    • 8
    Polyclonal
    Conjugate
    • 23
    • 9
    • 6
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This SIGLEC5 antibody is un-conjugated
    Application
    • 42
    • 25
    • 15
    • 6
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB)
    Purification
    This antibody is purified through a protein A column, followed by peptide affinity purification.
    Immunogen
    This SIGLEC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 482-513 amino acids from the C-terminal region of human SIGLEC5.
    Isotype
    Ig Fraction
    Top Product
    Discover our top product SIGLEC5 Primary Antibody
  • Application Notes
    For WB starting dilution is: 1:1000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied in PBS with 0.09 % (W/V) sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
  • Target
    SIGLEC5 (Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5))
    Alternative Name
    SIGLEC5 (SIGLEC5 Products)
    Background
    SIGLECs are members of the immunoglobulin superfamily that are expressed on the cell surface. Most SIGLECs have one or more cytoplasmic immune receptor tyrosine-based inhibitory motifs (ITIM). SIGLECs are typically expressed on cells of the innate immune system, with the exception of the B-cell expressed SIGLEC6. Sequence analysis predicted that the 697-amino acid SIGLEC10 protein contains a signal peptide, an N-terminal V-set Ig-like domain and four C2-set Ig-like domains, five potential N-linked glycosylation sites, a transmembrane region, and a 126-residue cytoplasmic tail with 3 putative ITIMs. Northern blot analysis detected a major 3.0-kb SIGLEC10 transcript, with highest levels in spleen, lymph node, blood leukocytes, and appendix. Little or no expression was observed in pancreas, thyroid, and testis. Flow cytometric analysis demonstrated eosinophil-specific expression of SIGLEC10, but at a lower level than that of SIGLEC8. Expression was also detected on monocytes and a CD16-positive/CD56-negative natural killer-like lymphocyte population. After sialidase treatment, which is necessary for unmasking the sialic acid-binding site on SIGLECs interacting with cell surface sialic acids, cells expressing SIGLEC10 bound to red blood cells. Immunoprecipitation analysis indicated expression of a 100- to 120-kD monomeric protein, higher than the predicted molecular mass, suggesting that SIGLEC10 is glycosylated.
    Molecular Weight
    61 kDa
    Gene ID
    8778
    UniProt
    O15389
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