MAP3K4 antibody (AA 1067-1097)

Catalog No. ABIN5534485

Product Details anti-MAP3K4 Antibody

Target: MAP3K4 (Mitogen-Activated Protein Kinase Kinase Kinase 4 (MAP3K4))

Binding Specificity: AA 1067-1097

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP3K4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This MEKK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1067-1097 amino acids from the Central region of human MEKK4.

Isotype: Ig Fraction

Application Details

Application Notes: For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for MAP3K4

Target: MAP3K4 (Mitogen-Activated Protein Kinase Kinase Kinase 4 (MAP3K4))

Alternative Name: MEKK4 (MAP3K4 Products)

Synonyms: MAPKKK4 antibody, MEKK4 antibody, MTK1 antibody, PRO0412 antibody, D17Rp17 antibody, D17Rp17e antibody, MEKK 4 antibody, Mek4b antibody, Mekk4 antibody, RP17 antibody, Rp17a antibody, Tas antibody, mKIAA0213 antibody, mitogen activated protein kinase kinase kinase 4 antibody, mitogen-activated protein kinase kinase kinase 4 antibody, Map3k4 antibody, MAP3K4 antibody

Background: The central core of each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. While the ERK MAPKs are activated by mitogenic stimulation, the CSBP2 and JNK MAPKs are activated by environmental stresses such as osmotic shock, UV irradiation, wound stress, and inflammatory factors. This gene encodes a MAPKKK, the MEKK4 protein, also called MTK1. This protein contains a protein kinase catalytic domain at the C terminus. The N-terminal nonkinase domain may contain a regulatory domain. Expression of MEKK4 in mammalian cells activated the CSBP2 and JNK MAPK pathways, but not the ERK pathway. In vitro kinase studies indicated that recombinant MEKK4 can specifically phosphorylate and activate PRKMK6 and SERK1, MAPKKs that activate CSBP2 and JNK, respectively but cannot phosphorylate PRKMK1, an MAPKK that activates ERKs. MEKK4 is a major mediator of environmental stresses that activate the CSBP2 MAPK pathway, and a minor mediator of the JNK pathway. Two alternatively spliced transcripts encoding distinct isoforms have been described.

Molecular Weight: 182 kDa

Gene ID: 4216

UniProt: Q9Y6R4

Pathways: MAPK Signaling