NEK4 antibody (C-Term)

Catalog No. ABIN5532956

Product Details anti-NEK4 Antibody

Target: NEK4 (NIMA related kinase 4 (NEK4))

Binding Specificity: AA 793-824, C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEK4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This NEK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 793-824 amino acids from the C-terminal region of human NEK4.

Isotype: Ig Fraction

Application Details

Application Notes: For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for NEK4

Target: NEK4 (NIMA related kinase 4 (NEK4))

Alternative Name: NEK4 (NEK4 Products)

Synonyms: NRK2 antibody, STK2 antibody, pp12301 antibody, ATNEK4 antibody, MAA21.6 antibody, NIMA-RELATED KINASE4 antibody, NIMA-related kinase 4 antibody, NIMA (never in mitosis gene a)-related expressed kinase 4 antibody, NIMA related kinase 4 antibody, NIMA-related kinase 4 antibody, Nek4 antibody, NEK4 antibody

Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

Molecular Weight: 95 kDa

Gene ID: 6787

UniProt: P51957