This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This HERC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-77 amino acids from the N-terminal region of human HERC3.
HERC3
Reactivity: Human
Host: Rabbit
Polyclonal
FITC
Application Notes
For WB starting dilution is: 1:1000
Restrictions
For Research Use only
Format
Liquid
Concentration
0.25 mg/mL
Buffer
Supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
5730409F18Rik antibody, AI646603 antibody, mKIAA0032 antibody, HECT and RLD domain containing E3 ubiquitin protein ligase 3 antibody, HECT and RLD domain containing E3 ubiquitin protein ligase 3 L homeolog antibody, probable E3 ubiquitin-protein ligase HERC3 antibody, hect domain and RLD 3 antibody, HERC3 antibody, herc3.L antibody, herc3 antibody, LOC100539754 antibody, LOC100563151 antibody, Herc3 antibody
Background
HERC3 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al., 2005 [PubMed 15676274]).