PVRL1 antibody (PE)

Catalog No. ABIN5526902

Product Details anti-PVRL1 Antibody (PE)

Target: PVRL1 (Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PVRL1 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: The mouse monoclonal antibody R1.302 recognizes an extracellular epitope on CD111 (also known as Nectin 1), a 75 kDa type I transmembrane glycoprotein broadly expressed on endothelial cells, epithelial cells, neuronal cells, megakaryocytes, and CD34-positive stem cells.

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: NIH/3T3 cells transfected with human CD111

Clone: R1-302

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Comment:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Handling

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details for PVRL1

Target: PVRL1 (Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1))

Alternative Name: CD111 (PVRL1 Products)

Background: Nectin cell adhesion molecule 1,CD111, also known as nectin-1, is a calcium-independent cell-cell adhesion transmembrane glycoprotein involved in organization of adherens junctions and tight junctions in epithelial and endothelial cells. It also serves as a target molecule for entry of herpes simplex virus (HSV-1, HSV-2) and pseudorabies virus (PRV) into epithelial and neuronal cells. CD111 is connected with actin cytoskeleton through afadin. Mutations in the gene for CD111 cause cleft lip and palate/ectodermal dysplasia 1 syndrome (CLPED1) as well as non-syndromic cleft lip with or without cleft palate (CL/P). Alternative splicing results in multiple transcript variants encoding proteins with distinct C-termini.,NECTIN1, ED4, HIgR, HV1S, HVEC, OFC7, PRR1, PVRR, CD111, PVRL1, PVRR1, SK-12, CLPED1

Gene ID: 5818

UniProt: Q15223

Pathways: Cell-Cell Junction Organization