MED18 antibody (AA 1-208)

Catalog No. ABIN5519036

Product Details anti-MED18 Antibody

Target: MED18 (Mediator Complex Subunit 18 (MED18))

Binding Specificity: AA 1-208

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED18 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for MED18 detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for MED18 detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.
Gene Name: mediator complex subunit 18
Protein Name: Mediator of RNA polymerase II transcription subunit 18

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived huamn MED18 recombinant protein (Position: M1-M208).

Isotype: IgG

Application Details

Application Notes: Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for MED18

Target: MED18 (Mediator Complex Subunit 18 (MED18))

Alternative Name: MED18 (MED18 Products)

Synonyms: p28b antibody, 2810046C01Rik antibody, mediator complex subunit 18 antibody, MED18 antibody, med18 antibody, Med18 antibody

Background: MED18 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II. Using in vitro-translated epitope-tagged proteins for protein-binding assays, it is found that MED18 directly interacted with the Mediator subunit TRFP (MED20). The MED18-TRFP heterodimer could also be coimmunoprecipitated from cotransfected insect cells. The MED18 gene was mapped to chromosome 1p35.3 based on an alignment of the MED18 sequence with the genomic sequence.

Synonyms: Mediator of RNA polymerase II transcription subunit 18, Mediator complex subunit 18, p28b, MED18

Gene ID: 54797

Pathways: Regulation of Lipid Metabolism by PPARalpha