MED13 antibody (AA 61-240)

Catalog No. ABIN5519033

Product Details anti-MED13 Antibody

Target: MED13 (Mediator Complex Subunit 13 (MED13))

Binding Specificity: AA 61-240

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED13 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for MED13 detection. Tested with WB, Direct ELISA in Human,Mouse.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for MED13 detection. Tested with WB, Direct ELISA in Human,Mouse.
Gene Name: mediator complex subunit 13
Protein Name: Mediator of RNA polymerase II transcription subunit 13

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived huamn MED13 recombinant protein (Position: L61-Y240).

Isotype: IgG

Application Details

Application Notes: Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for MED13

Target: MED13 (Mediator Complex Subunit 13 (MED13))

Alternative Name: MED13 (MED13 Products)

Synonyms: ARC250 antibody, DRIP250 antibody, HSPC221 antibody, THRAP1 antibody, TRAP240 antibody, 1110067M05Rik antibody, D030023K18 antibody, Thrap1 antibody, Trap240 antibody, med13 antibody, wu:fb66c06 antibody, wu:fc43h07 antibody, wu:fc43h08 antibody, zgc:153454 antibody, skuld-like antibody, mediator complex subunit 13 antibody, mediator complex subunit 13b antibody, MED13 antibody, Med13 antibody, med13b antibody

Background: Mediator complex subunit 13 is a protein that in humans is encoded by the MED13 gene. This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. The product of this gene is proposed to form a sub-complex with MED12, cyclin C, and CDK8 that can negatively regulate transactivation by mediator.

Synonyms: Mediator of RNA polymerase II transcription subunit 13, Activator-recruited cofactor 250 kDa component, ARC250, Mediator complex subunit 13, Thyroid hormone receptor-associated protein 1, Thyroid hormone receptor-associated protein complex 240 kDa component, Trap240, Vitamin D3 receptor-interacting protein complex component DRIP250, DRIP250, MED13, ARC250, KIAA0593, THRAP1, TRAP240

Gene ID: 9969

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Regulation of Lipid Metabolism by PPARalpha