MED4 antibody (AA 27-140)

Catalog No. ABIN5519029

Product Details anti-MED4 Antibody

Target: MED4 (Mediator Complex Subunit 4 (MED4))

Binding Specificity: AA 27-140

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for MED4 detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for MED4 detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.
Gene Name: mediator complex subunit 4
Protein Name: Mediator of RNA polymerase II transcription subunit 4

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived huamn MED4 recombinant protein (Position: R27-K140).

Isotype: IgG

Application Details

Application Notes: Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for MED4

Target: MED4 (Mediator Complex Subunit 4 (MED4))

Alternative Name: MED4 (MED4 Products)

Synonyms: zgc:110646 antibody, ARC36 antibody, DRIP36 antibody, HSPC126 antibody, TRAP36 antibody, VDRIP antibody, Vdrip antibody, 2410046H15Rik antibody, Vdirp antibody, mediator complex subunit 4 antibody, mediator complex subunit 4 S homeolog antibody, med4 antibody, med4.S antibody, MED4 antibody, Med4 antibody

Background: Mediator of RNA polymerase II transcription subunit 4, also known as mediator complex subunit 4 (MED4), a component of Mediator or vitamin D3 receptor-interacting protein complex 36 kDa component (DRIP36), is a protein that in humans is encoded by the MED4 gene. The Mediator complex interacts with DNA-binding gene-specific transcription factors to modulate transcription by RNA polymerase II. The MED4 gene was mapped to chromosome 13 based on sequence similarity between the MED4 sequence and a genomic contig.

Synonyms: Mediator of RNA polymerase II transcription subunit 4, Activator-recruited cofactor 36 kDa component, ARC36, Mediator complex subunit 4, TRAP/SMCC/PC2 subunit p36 subunit, Vitamin D3 receptor-interacting protein complex 36 kDa component, DRIP36, MED4, ARC36, DRIP36, VDRIP, HSPC126

Gene ID: 29079

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Regulation of Lipid Metabolism by PPARalpha