XDH antibody (AA 2-343)

Catalog No. ABIN5518967

Product Details anti-XDH Antibody

Target: XDH (Xanthine Dehydrogenase (XDH))

Binding Specificity: AA 2-343

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XDH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Xanthine dehydrogenase/oxidase(XDH) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): Predicted Cross Reactivity: human
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Xanthine dehydrogenase/oxidase(XDH) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: xanthine dehydrogenase
Protein Name: Xanthine dehydrogenase/oxidase

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Xanthine Oxidase recombinant protein (Position: T2-K343). Human Xanthine Oxidase shares 86.8% and 89.2% amino acid (aa) sequence identity with mouse and rat Xanthine Oxidase, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, Rat, Predicted Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Predicted Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-XDH antibody (ABIN5518967)

Huang, Zhu, Li, Sui, Min: "Effect of Blueberry Anthocyanins Malvidin and Glycosides on the Antioxidant Properties in Endothelial Cells." in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 1591803, (2017) (PubMed).

Target Details for XDH

Target: XDH (Xanthine Dehydrogenase (XDH))

Alternative Name: XDH (XDH Products)

Synonyms: XO antibody, XOR antibody, Xor antibody, Xox-1 antibody, Xox1 antibody, XDH antibody, im:7146167 antibody, DDBDRAFT_0219626 antibody, DDBDRAFT_0230176 antibody, DDB_0219626 antibody, DDB_0230176 antibody, xanthine dehydrogenase antibody, xanthine dehydrogenase/oxidase antibody, Xanthine dehydrogenase antibody, XDH antibody, Xdh antibody, xdh antibody, LOC576712 antibody, Trebr_1754 antibody

Background: Xanthine dehydrogenase, also known as XDH, is a protein that, in humans, is encoded by the XDH gene. Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism.

Synonyms: Xanthine dehydrogenase/oxidase, Xanthine dehydrogenase, XD

Gene ID: 7498

UniProt: P47989

Pathways: Positive Regulation of Endopeptidase Activity