FMN1 antibody (AA 1195-1419)

Catalog No. ABIN5518914

Product Details anti-FMN1 Antibody

Target: FMN1 (Formin 1 (FMN1))

Binding Specificity: AA 1195-1419

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FMN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Formin-1(FMN1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): Predicted Cross Reactivity: human
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Formin-1(FMN1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: formin 1
Protein Name: Formin-1

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human FMN1 recombinant protein (Position: N1195-N1419). Human FMN1 shares 93.8% amino acid (aa) sequence identity with mouse FMN1.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, Rat, Predicted Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Predicted Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for FMN1

Target: FMN1 (Formin 1 (FMN1))

Alternative Name: FMN1 (FMN1 Products)

Synonyms: si:dkey-81b15.10 antibody, fmn antibody, formin antibody, FMN antibody, LD antibody, BB164513 antibody, Fmn antibody, formin-1 antibody, ld antibody, RGD1306349 antibody, RGD1310100 antibody, formin 1 antibody, fmn1 antibody, FMN1 antibody, Fmn1 antibody

Background: Formins, such as FMN1, are actin-nucleating proteins involved in cell polarity, cytokinesis, cell migration, and transcriptional activity. This FMN1 gene belongs to the formin homology family and encodes a protein that has a role in the formation of adherens junction and the polymerization of linear actin cables. The homologous gene in mouse is associated with limb deformity. Alternatively spliced transcript variants have been found for this gene.

Synonyms: Formin-1, Limb deformity protein homolog, FMN1, FMN, LD

Gene ID: 342184

Pathways: Regulation of Actin Filament Polymerization