COPE antibody (AA 80-308)

Catalog No. ABIN5518902

Product Details anti-COPE Antibody

Target: COPE (Coatomer Protein Complex, Subunit epsilon (COPE))

Binding Specificity: AA 80-308

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COPE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Coatomer subunit epsilon(COPE) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Coatomer subunit epsilon(COPE) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: coatomer protein complex subunit epsilon
Protein Name: Coatomer subunit epsilon

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human COPE recombinant protein (Position: E80-A308). Human COPE shares 89.5% amino acid (aa) sequence identity with mouse COPE.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for COPE

Target: COPE (Coatomer Protein Complex, Subunit epsilon (COPE))

Alternative Name: COPE (COPE Products)

Synonyms: COPE antibody, DDBDRAFT_0189670 antibody, DDBDRAFT_0233799 antibody, DDB_0189670 antibody, DDB_0233799 antibody, DKFZp469K1716 antibody, MGC80063 antibody, MGC130714 antibody, epsilon-COP antibody, 1110005D17Rik antibody, Cope1 antibody, coatomer protein complex subunit epsilon antibody, coatomer protein complex epsilon subunit antibody, coatomer protein complex subunit epsilon S homeolog antibody, coatomer protein complex, subunit epsilon antibody, COPE antibody, copE antibody, cope.S antibody, cope antibody, Cope antibody

Background: Coatomer subunit epsilon is a protein that in humans is encoded by the COPE gene. The product of this gene is an epsilon subunit of coatomer protein complex. Coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles. It is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. Coatomer complex consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified.

Synonyms: Coatomer subunit epsilon, Epsilon-coat protein, Epsilon-COP, COPE

Gene ID: 11316

UniProt: O14579