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Thrombopoietin antibody (AA 22-259)

THPO Reactivity: Rat, Mouse WB, ELISA, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN5518876
  • Target See all Thrombopoietin (THPO) Antibodies
    Thrombopoietin (THPO)
    Binding Specificity
    • 6
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 22-259
    Reactivity
    • 42
    • 10
    • 9
    • 2
    • 1
    • 1
    • 1
    Rat, Mouse
    Host
    • 35
    • 10
    • 6
    • 6
    Rabbit
    Clonality
    • 48
    • 9
    Polyclonal
    Conjugate
    • 40
    • 7
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Thrombopoietin antibody is un-conjugated
    Application
    • 42
    • 27
    • 6
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Purpose
    Rabbit IgG polyclonal antibody for Thrombopoietin(Thpo) detection. Tested with WB, IHC-P, ELISA in Mouse,Rat.
    Cross-Reactivity (Details)
    No cross reactivity with other proteins.
    Characteristics
    Rabbit IgG polyclonal antibody for Thrombopoietin(Thpo) detection. Tested with WB, IHC-P, ELISA in Mouse,Rat.
    Gene Name: thrombopoietin
    Protein Name: Thrombopoietin
    Purification
    Immunogen affinity purified.
    Immunogen
    E. coli-derived mouse Thrombopoietin recombinant protein (Position: S22-H259). Mouse Thrombopoietin shares 79.3% and 92.9% amino acid (aa) sequence identity with human and rat Thrombopoietin, respectively.
    Isotype
    IgG
  • Application Notes
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse
    IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
    ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse

    Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
    Comment

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Concentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
  • Target
    Thrombopoietin (THPO)
    Alternative Name
    Thpo (THPO Products)
    Synonyms
    MGDF antibody, MKCSF antibody, ML antibody, MPLLG antibody, THCYT1 antibody, TPO antibody, Mgdf antibody, Ml antibody, Mpllg antibody, Tpo antibody, Tpo1 antibody, Tpo2 antibody, Tpo3 antibody, Tpo4 antibody, tpo antibody, LOC100231762 antibody, thrombopoietin antibody, THPO antibody, Thpo antibody, thpo antibody
    Background
    Thrombopoietin (THPO), also known as megakaryocyte growth and development factor (MGDF), is a protein that in humans is encoded by the THPO gene. Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent.

    Synonyms: C mpl ligand | MGDF | MKCSF | ML | MPL ligand | MPLLG | THCYT1 | THPO | Thrombopoietin | TPO | P40225
    Gene ID
    21832
    UniProt
    P40226
    Pathways
    JAK-STAT Signaling, Hormone Activity
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