HSD17B4 antibody (AA 510-736)

Catalog No. ABIN5518845

Product Details anti-HSD17B4 Antibody

Target: HSD17B4 (Hydroxysteroid (17-Beta) Dehydrogenase 4 (HSD17B4))

Binding Specificity: AA 510-736

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSD17B4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Peroxisomal multifunctional enzyme type 2(HSD17B4) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Peroxisomal multifunctional enzyme type 2(HSD17B4) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: hydroxysteroid (17-beta) dehydrogenase 4
Protein Name: Peroxisomal multifunctional enzyme type 2

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HSD17B4 recombinant protein (Position: D510-L736). Human HSD17B4 shares 87.7% and 89% amino acid (aa) sequence identity with mouse and rat HSD17B4, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for HSD17B4

Target: HSD17B4 (Hydroxysteroid (17-Beta) Dehydrogenase 4 (HSD17B4))

Alternative Name: HSD17B4 (HSD17B4 Products)

Synonyms: zgc:55545 antibody, zgc:77300 antibody, CG3415 antibody, DmMFE-2 antibody, Dmel\\CG3415 antibody, MFE-2 antibody, DBP antibody, MPF-2 antibody, PRLTS1 antibody, SDR8C1 antibody, 17-beta-HSD antibody, 17[b]-HSD antibody, 4 antibody, MFP2 antibody, Mfp-2 antibody, perMFE-2 antibody, hydroxysteroid (17-beta) dehydrogenase 4 antibody, hydroxysteroid 17-beta dehydrogenase 4 antibody, peroxisomal Multifunctional enzyme type 2 antibody, hsd17b4 antibody, HSD17B4 antibody, Mfe2 antibody, Hsd17b4 antibody

Background: Peroxisomal multifunctional enzyme type 2 is a protein that in humans is encoded by the HSD17B4 gene. The protein encoded by this gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathway for fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidation activity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present on chromosome 8. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Synonyms: 17 beta HSD 4 | 17 beta HSD IV | 17-beta-HSD 4 | 3-alpha | 7-alpha | DBP | EDH17B4 | Hsd17b4 | MFE2 | MFE-2 | MFE 2 | PRLTS1 | SDR8C1 | P51659

Gene ID: 3295

UniProt: P51659

Pathways: Monocarboxylic Acid Catabolic Process