Hemoglobin Alpha 1 + 2 (HBA1,HBA2) (AA 2-142) antibody

Catalog No. ABIN5518839

Product Details

Target: Hemoglobin Alpha 1 + 2 (HBA1,HBA2)

Binding Specificity: AA 2-142

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Hemoglobin subunit alpha(HBA1|HBA2) detection. Tested with WB, IHC-P in Human,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Hemoglobin subunit alpha(HBA1|HBA2) detection. Tested with WB, IHC-P in Human,Rat.
Gene Name: hemoglobin subunit alpha 1/2
Protein Name: Hemoglobin subunit alpha

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Hemoglobin recombinant protein (Position: V2-R142). Human Hemoglobin shares 85.8% amino acid (aa) sequence identity with mouse Hemoglobin.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details

Target: Hemoglobin Alpha 1 + 2 (HBA1,HBA2)

Alternative Name: HBA1|HBA2 (HBA1,HBA2 Products)

Synonyms: HBAM antibody, Hba-a1 antibody, Hba-a2 antibody, CD31 antibody, HBH antibody, Hba antibody, Hba1 antibody, Hbat1 antibody, hemoglobin, alpha 1 antibody, hemoglobin subunit alpha 1 antibody, hemoglobin alpha, adult chain 1 antibody, Hba1 antibody, HBA1 antibody, Hba-a1 antibody

Background: The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97 % of the total hemoglobin, alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3 % of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1, some nondeletion alpha thalassemias have also been reported.

Synonyms: CD31 | HBA 1 | HBA 2 | HBA | HBA T3 | HBA-T2 | HBA1 | HBA2 | Alpha-globin | P69905

Gene ID: 3039, 3040

UniProt: P69905