GJC1 antibody (N-Term)
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- Target See all GJC1 Antibodies
- GJC1 (Gap Junction Protein, gamma 1, 45kDa (GJC1))
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Binding Specificity
- AA 91-131, N-Term
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This GJC1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purpose
- Rabbit IgG polyclonal antibody for Gap junction gamma-1 protein(GJC1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
- Sequence
- YLGYAIHKIA KMEHGEADKK AARSKPYAMR WKQHRALEET E
- Cross-Reactivity (Details)
- No cross reactivity with other proteins.
- Characteristics
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Rabbit IgG polyclonal antibody for Gap junction gamma-1 protein(GJC1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: gap junction protein, gamma 1
Protein Name: Gap junction gamma-1 protein - Purification
- Immunogen affinity purified.
- Immunogen
- A synthetic peptide corresponding to a sequence at the N-terminus of human Connexin 45/GJA7 (91-131aa YLGYAIHKIAKMEHGEADKKAARSKPYAMRWKQHRALEETE), identical to the related mouse and rat sequences.
- Isotype
- IgG
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- Application Notes
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - Comment
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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Effect of Novel Gasotransmitter hydrogen sulfide on renal fibrosis and connexins expression in diabetic rats." in: Bioengineered, Vol. 7, Issue 5, pp. 314-320, (2017) (PubMed).
: "
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Effect of Novel Gasotransmitter hydrogen sulfide on renal fibrosis and connexins expression in diabetic rats." in: Bioengineered, Vol. 7, Issue 5, pp. 314-320, (2017) (PubMed).
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- Target
- GJC1 (Gap Junction Protein, gamma 1, 45kDa (GJC1))
- Alternative Name
- GJC1 (GJC1 Products)
- Synonyms
- CX45 antibody, GJA7 antibody, cx45 antibody, gja7 antibody, MGC52735 antibody, GJD3 antibody, GJC1 antibody, C130009G16Rik antibody, Cnx45 antibody, Cx45 antibody, Gja-7 antibody, Gja7 antibody, gap junction protein gamma 1 antibody, gap junction protein gamma 1 L homeolog antibody, gap junction protein, delta 3, 31.9kDa antibody, gap junction protein, gamma 1 antibody, Gap junction gamma-1 protein antibody, GJC1 antibody, gjc1.L antibody, GJD3 antibody, Gjc1 antibody, gjc1 antibody
- Background
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Gap junction gamma-1 protein (GJC1), also known as gap junction alpha-7 protein (GJA7) or connexin 45 (Cx45), is a protein that in humans is encoded by the GJC1 gene. The International Radiation Hybrid Mapping Consortium mapped the GJA7 gene to chromosome 17q21.31. This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell.
Synonyms: Gap junction gamma-1 protein | Connexin-45 | Cx45 | Gap junction alpha-7 protein | GJC1 | GJA7 | P36383 - Gene ID
- 10052
- UniProt
- P36383
- Pathways
- Cell-Cell Junction Organization
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