ALDH1A3 antibody (AA 37-154)

Catalog No. ABIN5518802

Product Details anti-ALDH1A3 Antibody

Target: ALDH1A3 (Aldehyde Dehydrogenase 1 Family, Member A3 (ALDH1A3))

Binding Specificity: AA 37-154

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALDH1A3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Aldehyde dehydrogenase family 1 member A3(ALDH1A3) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Aldehyde dehydrogenase family 1 member A3(ALDH1A3) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: aldehyde dehydrogenase 1 family member A3
Protein Name: Aldehyde dehydrogenase family 1 member A3

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ALDH1A3 recombinant protein (Position: N37-K154). Human ALDH1A3 shares 89% and 87.3% amino acid (aa) sequence identity with mouse and rat ALDH1A3, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for ALDH1A3

Target: ALDH1A3 (Aldehyde Dehydrogenase 1 Family, Member A3 (ALDH1A3))

Alternative Name: ALDH1A3 (ALDH1A3 Products)

Synonyms: RALDH3 antibody, ALDH1A3 antibody, ALDH1A6 antibody, ALDH6 antibody, MCOP8 antibody, Aldh6 antibody, V1 antibody, aldehyde dehydrogenase 1 family member A3 antibody, aldehyde dehydrogenase 1 family member A3 L homeolog antibody, aldehyde dehydrogenase 1 family, member A3 antibody, aldehyde dehydrogenase family 1, subfamily A3 antibody, ALDH1A3 antibody, aldh1a3.L antibody, aldh1a3 antibody, Aldh1a3 antibody

Background: Aldehyde dehydrogenase 1 family, member A3, also known as ALDH1A3 or retinaldehyde dehydrogenase 3 (RALDH3), is an enzyme that in humans is encoded by the ALDH1A3 gene. Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The enzyme encoded by this gene uses retinal as a substrate, either in a free or a cellular retinol-binding protein form. Mutations in this gene have been associated with microphthalmia, isolated 8, and expression changes have also been detected in tumor cells. Alternative splicing results in multiple transcript variants.

Synonyms: Aldh1a3 | ALDH1A6 | ALDH6 | RALDH 3 | V1 | P47895

Gene ID: 220

UniProt: P47895

Pathways: Retinoic Acid Receptor Signaling Pathway