DISC1 antibody (Middle Region)

Catalog No. ABIN5518699

Product Details anti-DISC1 Antibody

Target: DISC1 (Disrupted in Schizophrenia 1 (DISC1))

Binding Specificity: AA 461-477, Middle Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DISC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Disrupted in schizophrenia 1 protein(DISC1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: EKQQLQKEIE ALQARMF

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Disrupted in schizophrenia 1 protein(DISC1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: disrupted in schizophrenia 1
Protein Name: Disrupted in schizophrenia 1 protein

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human DISC1(461-477aa EKQQLQKEIEALQARMF), different from the related mouse sequence by two amino acids, and from the related rat sequence by four amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for DISC1

Target: DISC1 (Disrupted in Schizophrenia 1 (DISC1))

Alternative Name: DISC1 (DISC1 Products)

Synonyms: DISC1 antibody, C1orf136 antibody, SCZD9 antibody, disrupted in schizophrenia 1 antibody, disrupted in schizophrenia 1 protein antibody, disc1 antibody, DISC1 antibody, LOC100580606 antibody, Disc1 antibody

Background: DISC1(Disrupted in Schizophrenia 1), is a protein that is encoded by the DISC1 gene in humans. Ma et al.(2002) determined that the mouse Disc1 gene maps to chromosome 8 in a region with homology of synteny to human chromosome 1q42. Ozeki et al.(2003) demonstrated that rodent Disc1 expression displayed pronounced developmental regulation, with the highest levels in late embryonic life during development of the cerebral cortex. Millar et al.(2005) showed that DISC1 interacts with the UCR2 domain of phosphodiesterase-4B, implicated in susceptibility to schizophrenia, and that elevation of cellular cAMP leads to dissociation of PDE4B from DISC1 and in increase in PDE4B activity.

Synonyms: C1orf136 antibody|DISC1 antibody|DISC1_HUMAN antibody|Disrupted in schizophrenia 1 antibody|Disrupted in schizophrenia 1 protein antibody|KIAA0457 antibody|RP4-730B13.1 antibody|SCZD9 antibody

Pathways: Regulation of Cell Size