Spindlin 1 antibody (N-Term)

Catalog No. ABIN5516939

Product Details anti-Spindlin 1 Antibody

Target: Spindlin 1 (SPIN1)

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Cow, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Spindlin 1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: KTPFGKTPGQ RSRADAGHAG VSANMMKKRT SHKKHRSSVG PSKPVSQPRR

Predicted Reactivity: Cow: 100%, Dog: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against SPIN1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human SPIN1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Spindlin 1

Target: Spindlin 1 (SPIN1)

Alternative Name: SPIN1 (SPIN1 Products)

Synonyms: SPIN antibody, AA553322 antibody, AA673430 antibody, Spin antibody, spin antibody, spindlin antibody, MGC115436 antibody, Spindlin-1 antibody, DKFZp459K085 antibody, spindlin 1 antibody, spindlin 1 L homeolog antibody, SPIN1 antibody, Spin1 antibody, spin1 antibody, spin1.L antibody

Background: SPIN1 is a chromatin reader that specifically recognizes and binds histone H3 both trimethylated at 'Lys-4' and asymmetrically dimethylated at 'Arg-8' (H3K4me3 and H3R8me2a) and acts as an activator of Wnt signaling pathway dowstream of PRMT2. In case of cancer, promotes cell cancer proliferation via activation of the Wnt signaling pathway (PubMed:24589551). Overexpression induces metaphase arrest and chromosomal instability. Localizes to active rDNA loci and promotes the expression of rRNA genes (PubMed:21960006).It may play a role in cell-cycle regulation during the transition from gamete to embryo. Involved in oocyte meiotic resumption, a process that takes place before ovulation to resume meiosis of oocytes blocked in prophase I: may act by regulating maternal transcripts to control meiotic resumption.

Alias Symbols: SPIN1, OCR, SPIN,

Protein Interaction Partner: SUMO2, UBC, PRMT6, BAG3, NPM1, UBL4A, SMURF1, EIF4A3, YWHAE, MAGOH, GRK5, TCF3, ARRB2, ARRB1, ELAVL1, AGO3, PTN,

Protein Size: 262

Gene ID: 10927

NCBI Accession: NP_006708

UniProt: Q9Y657