Urm1 antibody (N-Term)

Catalog No. ABIN5516189

Product Details anti-Urm1 Antibody

Target: Urm1 (Ubiquitin Related Modifier 1 (Urm1))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Urm1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: TLPGQEEPWD IRNLLIWIKK NLLKERPELF IQGDSVRPGI LVLINDADWE

Characteristics: This is a rabbit polyclonal antibody against URM1. It was validated on Western Blot.

Purification: Affinity purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human URM1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Urm1

Target: Urm1 (Ubiquitin Related Modifier 1 (Urm1))

Alternative Name: URM1 (Urm1 Products)

Synonyms: RGD1306599 antibody, C9orf74 antibody, 2900073H19Rik antibody, ubiquitin related modifier 1 antibody, ubiquitin related modifier antibody, ubiquitin-related modifier antibody, Ubiquitin-related modifier 1 homolog antibody, Urm1 antibody, LOC733109 antibody, URM1 antibody

Background: URM1 acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). URM1 serves as sulfur donor in tRNA 2- thiolation reaction by being thiocarboxylated (-COSH) at its C- terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins such as MOCS3, ATPBD3, CTU2, USP15 and CAS. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.

Alias Symbols: C9orf74

Protein Interaction Partner: SEC23IP, DSTN, PTGES3, TXNL1, USP5, XPO1, VCL, PRDX2, PRDX1, ATP6V1A, CTU1, UBC, ASCC2, TBL3, CDK11B, UBA4,

Protein Size: 101

Gene ID: 81605

NCBI Accession: NP_112176

UniProt: Q9BTM9