MRPL50 antibody (N-Term)

Catalog No. ABIN5514549

Product Details anti-MRPL50 Antibody

Target: MRPL50 (Mitochondrial Ribosomal Protein L50 (MRPL50))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRPL50 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: CREFWSRFRK EKEPVVVETV EEKKEPILVC PPLRSRAYTP PEDLQSRLES

Predicted Reactivity: Cow: 85%, Dog: 79%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against RM50. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human RM50

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MRPL50

Target: MRPL50 (Mitochondrial Ribosomal Protein L50 (MRPL50))

Alternative Name: RM50 (MRPL50 Products)

Synonyms: ANON-65Eb antibody, BcDNA:GM06185 antibody, BcDNA:RE06140 antibody, CG8612 antibody, Dmel\\CG8612 antibody, MRP-L50 antibody, anon-65Eb antibody, DKFZp468B0910 antibody, AA408971 antibody, AI317351 antibody, D4Wsu125e antibody, mitochondrial ribosomal protein L50 antibody, mitochondrial 54S ribosomal protein MRPL50 antibody, mRpL50 antibody, Mrpl50 antibody, MRPL50 antibody

Background: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a putative 39S subunit protein and belongs to the L47P ribosomal protein family. Pseudogenes corresponding to this gene are found on chromosomes 2p, 2q, 5p, and 10q.

Alias Symbols: MRPL50,

Protein Interaction Partner: UBC, MDM2, NPM1, MRPL45, TMEM177, MRPL57, MRPL44, MRPL32, MRPL38, MRPL40, MRPS9, MRPL47, TSR1, RIF1, MRPL37, MRPL15, FLOT1, MRPL19, SUCLG2, SLC25A3, ILF2, ATP6V0A1, ATP6V1B1, CUL3, ICT1,

Protein Size: 158

Gene ID: 54534

NCBI Accession: NP_061924

UniProt: Q8N5N7