RPSA antibody (C-Term)
-
- Target
- RPSA (30S Ribosomal Protein S1 (S1) (RPSA))
- Binding Specificity
- C-Term
- Reactivity
- Synechocystis PCC 6803
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Western Blotting (WB)
- Characteristics
- Expected / apparent Molecular Weight of the Antigene: 35 kDa
- Purification
- serum
- Immunogen
- recombinant S1 protein of Synechocystis sp. strain PCC6803, one Flag-tag sequence (N-DYKDDDDK-C) was added to the C-terminus of the protein P73530
-
- Application Notes
- Recommended Dilution: 1 : 1000 with standard ECL (WB).
- Comment
-
For immunogold experiments plant tissue has been fixed with GA in PFA/PIPES.LR White resin has been used. Tested species were: Triticum aestivum, Panicummiliaceum, Panicum maximum, Echinochloa crus-galli Eragrostis neomexicana,Digitaria sanguinalis.
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- For reconstitution add 200 µL of sterile water
- Handling Advice
-
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. - Storage
- -20 °C
-
- Target
- RPSA (30S Ribosomal Protein S1 (S1) (RPSA))
- Alternative Name
- 30S ribosomal protein S1 (S1)
- Synonyms
- ssyF antibody, 30S ribosomal protein S1 antibody, rpsA antibody, RB2543 antibody, PMEN_RS09310 antibody
- Background
- S1 ribosomal protein is involved in initiation of translation by recognition and binding of mRNAs through association with the 30S ribosomal subunit. S1 protein is localized in bacterial cytosol.
- Molecular Weight
- 35 kDa
- UniProt
- P73530
-